Regulatory
Part:BBa_K4317001
Designed by: Chris Song Group: iGEM22_Seoul_Korea (2022-10-11)
P2069M
P2069 originated from B. pumilus BA06. P2069M, engineered by Cui-Cui Miao and colleagues, has strong promoter activity in Bacillus subtilis.
Usage and Biology
Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3)
References
Miao, Cui-Cui, et al. "Construction of a high-expression system in Bacillus through transcriptomic profiling and promoter engineering." Microorganisms 8.7 (2020): 1030.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7
Illegal BglII site found at 13
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |